Enzymatic method for producing 2S,4R-monatin

The present invention provides a methodology for improving a yield of 2R, 4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form th...

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Bibliographic Details
Main Authors SUGIYAMA, MASAKAZU, ISHIKAWA, KOKI, TAKAKURA, YASUAKI, MORI, KENICHI, TABUCHI, ERI, TAGAMI, UNO, OGINO, HIROOMI, FUJII, HIDEMI
Format Patent
LanguageEnglish
French
German
Published 25.07.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a methodology for improving a yield of 2R, 4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S, 4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S, 4R-Monatin to form the 2R, 4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.
Bibliography:Application Number: EP20110820789