Methods for producing succinic acid

• Main Authors: CALANDE, OLIVIER, VARLAMOFF, CAROLINE, SEGUEILHA, LAURENT, DEHAY, FREDERIC
• Format: Patent
• Language: English; French; German
• Published: 29.02.2012
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; SPIRITS; VINEGAR; WINE

Producing succinic acid by anaerobic fermentation of a carbon source with an Escherichia colistrain comprises a first stage in which carbon dioxide is supplied in an amount dependent on its consumption by the strain and, before total exhaustion of the carbon source, a second step in which the remaining carbon source is fermented without supplying carbon dioxide so as to consume residual carbon dioxide. An independent claim is also included for producing succinic acid by anaerobic fermentation with an Escherichia colistrain in the presence of carbon dioxide while adding a magnesium compound to the fermentation medium to adjust the pH, where the resulting magnesium succinate is then converted to succinic acid. L'invention concerne un procédé de production d'acide succinique, comprenant : (a) une étape de culture d'une souche d'Escherichia coli en conditions aérobies, lors de laquelle le pH est régulé par ajout, dans le milieu de culture, d'un composé de magnésium, (b) une étape de production d'ions succinate par fermentation de la souche cultivée à l'étape (a) en conditions anaérobies en présence de CO 2 , (c) une étape de conversion des ions succinate formés à l'étape (b) en acide succinique.