METHOD AND DEVICE FOR MEASURING THE ACTIVITY OF ENZYMES AFTER INHIBITOR REMOVAL

• Main Authors: MEIER, HANS JÖRG, HOFER, HANS WERNER
• Format: Patent
• Language: English; French; German
• Published: 04.11.2015
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention relates to a method for measuring the activity of enzymes in a sample which contains at least one enzyme and at least one enzyme inhibitor corresponding to said enzyme, whereby after de-inhibition the activity of the released enzyme is measured in such a way that a substrate is added to the sample and the time course of the concentration of at least one reaction product (cleavage product) is recorded and the enzyme specific substrate has a fluorogenic part which is cleaved in the enzymatic reaction and the fluorescence (measuring parameter) of which can be detected in a wavelength range where the measuring parameter can be assigned unambiguously to the enzyme activity to be measured, whereby the de-inhibition is carried out by immersing a rigid carrier, to which the inhibitor binding substance is bound, into the sample. The present invention relates also to a corresponding device for measuring the activity on enzymes in a sample.