Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells

• Main Authors: MERETSKI, SHAI, KADOURI, AVINOAM, ZIPORI, DOV, MERCHAV, SHOSHANA
• Format: Patent
• Language: English; French; German
• Published: 06.04.2016
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BANDAGES, DRESSINGS OR ABSORBENT PADS; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF,TUBULAR STRUCTURES OF THE BODY, E.G. STENTS; ENZYMOLOGY; FILTERS IMPLANTABLE INTO BLOOD VESSELS; FIRST-AID KITS; FOMENTATION; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PROSTHESES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TREATMENT OR PROTECTION OF EYES OR EARS; VINEGAR; WINE

The present invention concerns a method of preparing a conditioned medium from a confluent stromal cell culture, the method comprising: (a) culturing stromal cells to confluence in a stationary phase plug-flow bioreactor under continuous flow of medium on a three dimensional substrate to obtain a stationary phase three-dimensional confluent stromal cell culture, wherein said stromal cell culture is grown to a density of at least 5 x 10 6 cells per a cubic centimeter of said substrate; and (b) separating and collecting a stromal cell conditioned medium from said stationary phase confluent stromal cell culture; thereby obtaining the conditioned medium.