Oligonucleotide library encoding randomised peptides

• Main Authors: HINE, ANNA, VICTORIA, HUGHES, MARCUS, DANIEL, ASHRAF, MOHAMMED
• Format: Patent
• Language: English; French; German
• Published: 30.11.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a kit for producing an oligonucleotide library, the library comprising a plurality of oligonucleotides, each oligonucleotide having at least one predetermined position, a randomisation codon selected from a defined group of codons, the coding within the defined group coding for different amino acids, the kit comprises a plurality of different randomisation oligonucleotides comprising: (i) a coding strand, the coding strand comprising a randomisation codon; and (ii) a substantially complementary non-coding strand, wherein each double stranded randomisation oligonucleotide comprises a nucleotide sequence coding for a restriction endonuclease recognition site capable of being recognised by a restriction endonuclease, the restriction endonuclease capable of cleaving the randomisation oligonucleotide upstream or downstream of the endonuclease recognition site at a predetermined cleavage site to create a blunt ended cut.