Apparatus for synthesis of arrays of DNA probes

• Main Authors: Sussman, Michael, R, Cerrina, Francesco, Green, Roland, Blattner, Frederick, R, Singh-Gasson, Sangeet
• Format: Patent
• Language: English; French; German
• Published: 01.11.2017
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS SPECIALLY ADAPTED THEREFOR; APPARATUS THEREFOR; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; CINEMATOGRAPHY; COMBINATORIAL CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DERIVATIVES THEREOF; ELECTROGRAPHY; ENZYMOLOGY; GENERAL METHODS OF ORGANIC CHEMISTRY; HOLOGRAPHY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MATERIALS THEREFOR; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; ORIGINALS THEREFOR; PERFORMING OPERATIONS; PHOTOGRAPHY; PHOTOMECHANICAL PRODUCTION OF TEXTURED OR PATTERNED SURFACES,e.g. FOR PRINTING, FOR PROCESSING OF SEMICONDUCTORDEVICES; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; SUGARS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING; USE OF INORGANIC OR NON-MACROMOLECULAR ORGANIC SUBSTANCES ASCOMPOUNDING INGREDIENTS; VINEGAR; WINE

The synthesis of arrays of DNA probe sequences, polypeptides and the like is carried out using a patterning process on an active surface of a substrate (12). An image is projected onto the active surface (15) utilizing an image former (11) that includes a light source that provides light to a micromirror device (35) comprising an array of electronically addressable micromirrors (36). The substrate (12) is activated in a defined pattern and bases are coupled at the activated sites, with further repeats until the elements of a two-dimensional array on the substrate have an appropriate base bound thereto. The micromirror array (35) can be controlled in conjunction with a DNA synthesizer to control the sequencing of images presented by the micromirror array (35) in coordination with the reagents provided to the substrate (12).