Growth medium for Clostridium histolyticum without ingredients of mammalian sources

The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferablyplant-...

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Bibliographic Details
Main Authors SUPPMANN, BERNHARD, HOFFMANN, ARTUR, SONN, KIRSTEN, MARX, THOMAS, THALHOFER, JOHANN-PETER, HOELKE, WERNER
Format Patent
LanguageEnglish
French
German
Published 16.12.2009
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferablyplant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.
Bibliography:Application Number: EP20090007502