METHOD OF DETECTING TARGET NUCLEIC ACID AND CONTAINER USED IN THE DETECTION METHOD

• Main Authors: TANIGAMI, YASUO, HASHIDO, KENTO
• Format: Patent
• Language: English; French; German
• Published: 11.11.2009
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

An object is to provide a method of easily detecting a target nucleic acid with high precision without using a hybridization method and a container used for the detecting method. The method of detecting the target nucleic acid 30, in which a first ligand 31 and a second ligand 32, which are ligands of different kinds, are bonded to a nucleic acid of a detection object, includes steps of preparing a first particle 33 bonded with a plurality of first receptors 331 to which the first ligand 31 specifically binds and a second particle 34 bonded with a plurality of second receptors 341 to which the second ligand 32 specifically binds, mixing a sample which may have the target nucleic acid 30, the first particle 33 and the second particle 34 in a container having a precipitation capturing part on a bottom thereof, and observing a capturing distribution of agglomerates including the target nucleic acid 30 with the first particle 33 and the second particle 34 where the particles are linked through the bonding between the first ligand and the first receptor and the bonding between the second ligand and the second receptor, respectively, and/or a sediment of the first particle 33 and the second particle 34, on the bottom of the container, after the mixing step.