PSEUDOTRANSPOSONS AND RECOMBINANT HYPERACTIVE TRANSPOSITION SYSTEMS, DERIVED FROM THE MOS-1 TRANSPOSON

The invention concerns a system for transposing a hyperactive recombinant derivative of Mos-1 transposon, comprising at least the two following partners: a) a Mos-1 pseudo-transposon in which an exogenous nucleotide sequence of interest replaces the nucleotide sequence encoding the original Mos-1 tr...

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Bibliographic Details
Main Authors BONNIN-ROULEUX, FLORENCE, GERMON, STEPHANIE, ODILE, REBECCA, BIGOT, YVES, PHILIPPE, MARCEL, JEGOT, GWENHAEL, BIGOT, SYLVIE, MARIE-LOUISE
Format Patent
LanguageEnglish
French
German
Published 11.02.2009
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention concerns a system for transposing a hyperactive recombinant derivative of Mos-1 transposon, comprising at least the two following partners: a) a Mos-1 pseudo-transposon in which an exogenous nucleotide sequence of interest replaces the nucleotide sequence encoding the original Mos-1 transposase; and b) a Mos-1 tranposase provided in trans in said pseudo-transposon, at least one of said partners being appropriately genetically modified to improve the transposition frequency of said exogenous nucleotide sequence of interest. Additionally to such systems, the invention concerns hyperactive Mos-1 transposons, hyperactive Mos-1 transposases, kits. The invention further concerns the use of one or more of abovementioned means for carrying out sequence transpositions, and more particularly efficient gene transfers.
Bibliography:Application Number: EP20070731461