SEPARATION METHOD OF NUCLEATED CELLS DERIVED FROM BONE MARROW FOR BONE FORMATION
The present invention relates to a method for separating bone marrow-derived nucleated cells for bone formation. The method comprises: the steps of: washing bone marrow; lysing red blood cells in the bone marrow; neutralizing the bone marrow lysate; purifying nucleated cells from the neutralized bon...
Saved in:
Main Authors | , , , |
---|---|
Format | Patent |
Language | English French German |
Published |
04.05.2016
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The present invention relates to a method for separating bone marrow-derived nucleated cells for bone formation. The method comprises: the steps of: washing bone marrow; lysing red blood cells in the bone marrow; neutralizing the bone marrow lysate; purifying nucleated cells from the neutralized bone marrow; and mixing the nucleated cells with a maintenance buffer for bone formation. According to the invention, bone marrow-derived nucleated cells, which can be grafted into a site in need of bone formation or bone defect treatment, can be separated in a rapid and convenient manner. As a result, bone formation in emergency patients or patients, who require repeated surgical operations, can be effectively achieved by separating the nucleated cells for bone formation in surgical locations in a convenient and rapid manner and injecting the separated cells into the patients. |
---|---|
Bibliography: | Application Number: EP20060769014 |