METHOD OF EVALUATING CELL FUNCTION, SYSTEM FOR EVALUATING CELL FUNCTION, FLUORESCENT MICROSCOPE SYSTEM, PHOTOTHERAPY METHOD AND PHOTOTHERAPY SYSTEM

A proposition of the present invention is to accurately evaluate a phototoxic property (cell function) concerning a living cell. To this end, an evaluating method of cell function of the present invention includes the operations of dyeing a specific site (41) of the living cell (40) with a fluoresce...

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Bibliographic Details
Main Authors SAKURAI, TAKASHI, IKI, YOICHI
Format Patent
LanguageEnglish
French
German
Published 17.12.2008
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:A proposition of the present invention is to accurately evaluate a phototoxic property (cell function) concerning a living cell. To this end, an evaluating method of cell function of the present invention includes the operations of dyeing a specific site (41) of the living cell (40) with a fluorescent dye, irradiating the living cell (40) with light to measure changes in brightness of resulting fluorescence generated at an adjacent site (43) of the specific site (41), and evaluating the phototoxic property based on the brightness changes. In the event of functional depression in the specific site (41), the fluorescent dye cannot be retained therein and is extravasated into the adjacent site (43) through the membrane of the specific site. The present invention can measure the extent of this extravasation, making it possible to accurately evaluate the phototoxic property.
Bibliography:Application Number: EP20070736970