P-ET-TS expression units in CORYNEBACTERIUM GLUTAMICUM

• Main Authors: ZELDER, OSKAR, KROEGER, BURKHARD, SCHROEDER, HARTWIG, KLOPPROGGE, CORINNA, HAEFNER, STEFAN
• Format: Patent
• Language: English; French; German
• Published: 02.11.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

A specific nucleic acid (I) with promoter activity is used for transcribing genes, where (I) is: (a) a sequence (1) of 178 nucleotides (reproduced); (b) a derivative of (1) with >= 90% identity, formed by substitution, insertion or deletion; (c) a sequence that hybridizes to (1) under stringent conditions; or (d) a functional fragment of (a)-(c). Independent claims are also included for: (1) use of an expression unit (EU), containing and linked to a nucleic acid (NA) that ensures translation of RNA, for expressing genes; (2) (I), except sequence (1) itself, as new compounds and EU containing it; (3) method for altering (or causing) the transcription (or expression) rate of genes in microorganisms relative to the wild type; (4) expression cassette (EC) comprising EU, at least one other functionally linked NA to be expressed and optionally other genetic control elements that are heterologous with respect to EU; (5) expression vector containing EC; (6) genetically modified microorganism (GMM) in which the transcription rate of at least one gene is altered (or caused) relative to the wild type; (7) GMM containing EU and a functionally linked gene to be expressed, where this is heterologous with respect to EU; (8) preparation of biosynthetic products (A) by culturing the GMM of (6) or (7); (9) use of the sequence aggagga (21) as ribosome-binding site in expression units for expressing genes in Corynebacterium or Brevibacterium; (10) use of the sequences ttaatt (19) or taagct (20) as -10 regions in EU for expressing genes in Corynebacterium or Brevibacterium; and (11) EU that contain (21) or at least one of (19) or (20).