ASSAYS BASED ON LIQUID FLOW OVER ARRAYS

Cassette ( 50 ) performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette ( 50 ), flowing over wide array ( 20 ) of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1x10-1 to 5x1...

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Bibliographic Details
Main Authors DOWD, ROGER, MAIMONIS, PETER, RODIONOVA, NATALIA, MONTAGU, JEAN, DEWEERD, HERMAN, TYBURCZY, NATHAN
Format Patent
LanguageEnglish
French
German
Published 20.02.2008
Subjects
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
TECHNICAL SUBJECTS COVERED BY FORMER USPC
TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
TESTING
TRANSPORTING
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Summary:Cassette ( 50 ) performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette ( 50 ), flowing over wide array ( 20 ) of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1x10-1 to 5x10-3, are heated in region ( 34 ) preceding and including bubble removal system ( 128 ). Analyte is introduced through compressed septum ( 32 ). External actuations of displacement pumps ( 30, 37 ) and valves ( 137 A, B, and C) produce flows in response to flow-front optical sensors ( 150, 152 ). Elastic sheet provides pump and valve diaphragms and resilient expansion of mixing volume ( 131 ). Break-away cover portions are pistons. Heating is by conduction through cassette from external contact heater. Planar cassette body, when tilted from horizontal, enables upward flow from pumped storage ( 134, 135 ) to reaction ( 133 ) to waste ( 139 ), with buoyancy bubble removal before reaction. Reading of fluorescence is by external reader, employing calibration, control and reference features on capture surface. Extensive set of calibration features of differing intensities enables self-calibration.
Bibliography:Application Number: EP20050824601