METHODS AND MATERIALS FOR OPTIMIZATION OF ELECTRONIC TRANSPORTATION AND HYBRIDIZATION REACTIONS
Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electroph...
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Main Authors | , , , , , |
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Format | Patent |
Language | English French German |
Published |
13.06.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent. |
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Bibliography: | Application Number: EP20050802166 |