Alleles of the mtK gene from coryneform bacteria

• Main Authors: SCHISCHKA, NATALIE, BATHE, BRIGITTE
• Format: Patent
• Language: English; French; German
• Published: 24.05.2006
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Replicable DNA sequence (I) derived from coryneform bacteria, coding for an S-adenosylmethionine synthetase polypeptide with an amino acid sequence in which valine at position 200 or isoleucine at position 322 of a defined sequence (SEQ ID No.2) is replaced by another proteinogenic amino acid, is new : Independent claims are also included for: (1) plasmid vectors containing (I); (2) coryneform bacteria containing (I); (3) bacteria containing a vector as above; (4) isolated coryneform bacteria containing nucleic acid sequences coding for a polypeptide as above which is 4 amino acids longer than the wild type protein and has the sequence Ala-Gln-Pro-Ser-Ser-Cys-Pro-Lys-Asn-Leu-Met at positions 401-411; (5) producing L-methionine or animal feed additives containing L-methionine by culturing coryneform bacteria in which alleles of the endogenous metK gene are overexpressed under conditions suitable for forming the metK gene product S-adenosylmethionine synthetase and isolating the L-methionine or animal feed additive from the culture broth; (6) producing L-methionine or animal feed additives containing L-methionine by culturing coryneform bacteria having an endogenous nucleic acid sequence of type (I), enriching L-methionine in the culture broth and isolating the L-methionine or animal feed additive from the culture broth.