USE OF ALPHA 1-ANTICHYMOTRYPSIN POLYPEPTIDES, OR NUCLEIC ACIDS ENCODING THEM, IN COMBINATION WITH ALPHA 1-ANTITRYPSIN POLYPEPTIDES, OR NUCLEIC ACIDS ENCODING THEM, FOR TREATMENT AND/OR PREVENTION OF DIABETES-ASSOCIATED AND/OR ARTERIAL POORLY HEALING WOUNDS

• Main Authors: GOPPELT, ANDREAS, HALLE, JOERN-PETER
• Format: Patent
• Language: English; French; German
• Published: 03.05.2006
• Edition: 7
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

Using alpha 1-antichymotrypsin (ACT) polypeptide, its functional variant and/or encoding nucleic acid, or of a cell expressing ACT polypeptide or its encoding nucleic acid, in combination with alpha 1-antitrypsin (AAT) polypeptide, its functional variant or encoding nucleic acid, or with a cell expressing AAT polypeptide or its encoding nucleic acid, for treating and/or preventing poorly healing diabetes-associated and/or arterial wounds, is new. An independent claim is also included for a method of manufacturing a pharmaceutical composition for treating and/or preventing the diseases described above where the ACT polypeptide or its encoding nucleic acid or a cell expressing the ACT polypeptide or its encoding nucleic acid is combined with the AAT polypeptide or its encoding nucleic acid or a cell expressing the AAT polypeptide or its encoding nucleic acid. ACTIVITY : Vulnerary; Antiulcer; Antidiabetic. Diabetes was induced in male Sprague Dawley rats of 250-300 g body weight by injecting intraperitoneally with streptozotocin. The backs of the 4 diabetic and 4 nondiabetic control animals were depilated and 4 sites were marked for subsequent wounding. Plasmid DNA (0.5 Microg) was shot into each site with in each case 2 sites being bombarded with the ACT expression vector pMHIntACT and 2 sites being bombarded with the control vector pMHIntLuc. The tensile strength of the wounds treated with pMHIntACT was clearly increased in the diabetic animals. Administration of the plasmid had no significant effect in the control animals. MECHANISM OF ACTION : Gene therapy; Protein therapy.