Lateral-flow measuring device and method for the measurement of analytes

• Main Authors: ZWINGMANN, DETLEV, MEISEGEIER, BERNHARD, SCHAPER, REINHARD, OBERSTRASS, JUERGEN
• Format: Patent
• Language: English; French; German
• Published: 08.08.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Device (I), for detecting and/or determining the concentration of at least an analyte in a fluid medium, comprises rigid, reaction- and detection area (50), that is continuous along the carrier, and is formed by a oblong support (52) with a cover (54); and a fluid receiving element (60) arranged below the reaction- and detection area. Device (I), for detecting and/or determining the concentration of at least an analyte in a fluid medium (obtained from: a stripe shaped carrier (20) with two longitudinal folds (24) on which several repeating printed electrode are bound from at least one reference electrode (30) and from at least a working electrode (40); and a ligand that is suited for binding to a biological active specific binding molecule or the analyte), comprises rigid, reaction- and detection area (50), that is continuous along the carrier, and is formed by a oblong support (52) with a cover (54); and a fluid receiving element (60) arranged below the reaction- and detection area. Independent claims are also included for: (1) the preparation of (I); and (2) a method for detecting and determining the concentration of dissolved analytes in a fluid medium, using (I), comprises applying the fluid medium on the sample zone, allowing the analyte to interact, for a time period, with the ligand and/or biologically active specific binding molecule and/or catalytically active protein, applying a solution (comprising at least a reaction specific substrate for one or more catalytically active protein) on the sample zone, connecting (I) to an electrically measuring instrument and determining the reading from the signal obtained in the measuring instrument after incubating and calibrating a curve for the concentration of the respective analytes.