METHOD FOR CHROMOSOMAL ENGINEERING

A method is provided for the integration of foreign genetic elements into a bacterial chromosome without the need of a cloning step. The method relies on the presence of the lambda-Red recombinase system in a recombination proficient bacterial host. At least two linear recombination elements or cons...

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Bibliographic Details
Main Author SUH, WONCHUL
Format Patent
LanguageEnglish
French
German
Published 14.09.2005
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method is provided for the integration of foreign genetic elements into a bacterial chromosome without the need of a cloning step. The method relies on the presence of the lambda-Red recombinase system in a recombination proficient bacterial host. At least two linear recombination elements or constructs are co-transformed into the recombination proficient host which are assembled in the correction orientation in the bacterial chromosome by homologous recombination. Selectable markers used for the selection of transformants are later excised by the action of a site-specific recombination.
Bibliography:Application Number: EP20030810091