Methods for isolating nucleic acids

• Main Authors: WEINDEL, KURT, SAROFIM, EMAD, ZIELENSKI, RALF, KIRCHGESSER, MICHAEL, WALTER, THOMAS, BERGMANN, FRANK
• Format: Patent
• Language: English; French; German
• Published: 04.03.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The problem underlying the present invention is to provide an alternative method for the chromatographic purification of a nucleic acid using alternative substances in the aequous adsorption solution, in order to facilitate the binding of nucleic acids to a substrate such as a mineral support. A method is provided for the purification of a nucleic acid, comprising the steps of: a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aequous buffer, (ii) salts in a high concentration and (iii) a water-miscible non-acidic organic compound, and (iv) the nucleic acid; b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid, followed by c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate, and d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid, and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.