METHOD OF GENE AMPLIFICATION

A method of gene amplification which comprises annealing primers to a target gene of an animal, a plant, etc. and then amplifying the gene by using a heat-resistant nucleic acid polymerase such as Taq polymerase or Pfu polymerase, characterized by using at least one high-pressure fluid selected from...

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Bibliographic Details
Main Author MISHIMA, KENJI
Format Patent
LanguageEnglish
French
German
Published 23.07.2003
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:A method of gene amplification which comprises annealing primers to a target gene of an animal, a plant, etc. and then amplifying the gene by using a heat-resistant nucleic acid polymerase such as Taq polymerase or Pfu polymerase, characterized by using at least one high-pressure fluid selected from the group consisting of supercritical fluids such as carbon dioxide, subcritical fluids, high-pressure gases and liquids as the reaction medium. It is preferable to amplify the gene by using a surfactant-coated enzyme obtained by coating a heat-resistant nucleic acid polymerase with a surfactant and controlling the reaction temperature.
Bibliography:Application Number: EP20010980933