METHOD AND KIT FOR THE MANUFACTURE OF SEPARATION GELS

• Main Authors: STIGH, LENA, PALMGREN, RONNIE
• Format: Patent
• Language: English; French; German
• Published: 11.10.2006
• Subjects: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS; MEASURING; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; SEPARATION; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS; SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING

A method for the manufacture of a separation gel, which comprises the steps: (i) providing a polymerization mixture containing a first monomer (I) having a polymerizable unsaturated structure and a second monomer (II) comprising two or more polymerizable unsaturated structures and an initiator, and (ii) polymerizing the mixture. The method is characterized in that monomer (I) is an N-vinyl carboxamide; and that (a) the initiating system for the polymerization comprises a UV photoinitiator and polymerization is initiated by UV irradiating the mixture at a wave-length appropriate for the initiator, and/or (b) preselecting monomer (I) and monomer (II) to have comparable reactivities to become incorporated into the growing polymer. A kit for use in the production of an electrophoretic gel is also described. The kit comprises: (a) a monomer (I) carrying an N-vinyl carboxamide group; (b) a monomer (II) carrying a polymerizable unsaturated structure; and (c) an UV initiator compound, a buffer and/or a denaturing agent; said monomers (I and II) being selected to provide comparable reactivities.