METHOD FOR PRODUCING ACTIVE SERINE PROTEASES AND INACTIVE VARIANTS

A method for producing biologically active serine proteases, isolated serine protease domains and their amino acid variants in a prokaryotic host is disclosed. The method comprises an N-terminal addition of a helper sequence consisting of a dipeptide which is suitable for degradation by a dipeptidyl...

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Bibliographic Details
Main Authors BODE, WOLFRAM, PARRY, MARINA, A., A, WILHARM, ELKE, JENNE, DIETER, HUBER, ROBERT
Format Patent
LanguageEnglish
French
German
Published 19.06.2002
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method for producing biologically active serine proteases, isolated serine protease domains and their amino acid variants in a prokaryotic host is disclosed. The method comprises an N-terminal addition of a helper sequence consisting of a dipeptide which is suitable for degradation by a dipeptidyl amino peptidase, by the expression of the serine protease(s) and/or its (their) fragments containing the N-terminal dipeptide helper sequence, optionally as inclusion bodies, and by the renaturation of the expressed proteins and the activation of the serine protease(s) and/or serine protease domains by splitting off the helper sequence using an exopeptidase.
Bibliography:Application Number: EP20000964151