MALDI ION SOURCE WITH A PULSE OF GAS, APPARATUS AND METHOD FOR DETERMINING MOLECULAR WEIGHT OF LABILE MOLECULES

A mass spectrometer instrument for determining the molecular weight of labile molecules of biological importance, in particular heavy molecules, such as proteins, peptides or DNA oligomers, is disclosed. The instrument includes a MALDI ion source that is enclosed in a chamber with an inlet for admit...

Full description

Saved in:
Bibliographic Details
Main Authors VESTAL, MARVIN, L, VERENTCHIKOV, ANATOLI, N, SMIRNOV, IGOR, P
Format Patent
LanguageEnglish
French
German
Published 27.04.2011
Subjects
BASIC ELECTRIC ELEMENTS
ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
ELECTRICITY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online AccessGet full text

Cover

More Information
Summary:A mass spectrometer instrument for determining the molecular weight of labile molecules of biological importance, in particular heavy molecules, such as proteins, peptides or DNA oligomers, is disclosed. The instrument includes a MALDI ion source that is enclosed in a chamber with an inlet for admitting a gas and an ion sampling aperture for limiting gas flow from the chamber. The elevated pressure of the source in the range from 0.1 to 10 torr causes low energy collisions between the gas and the ions that can cause rapid collisional cooling of the excited ions, thereby improving the stability of the produced ions. The formation of clusters of ions (e.g., protein ions) with matrix materials is broken without fragmenting the ions by increasing the downstream gas temperature to between 150 and 250° C. Operating the source at laser energy at least two times higher than the threshold value of ion formation and using a high repetition rate laser significantly improves sensitivity of analysis and speed of data acquisition.
Bibliography:Application Number: EP20000939394