Nuclease

A strand-specific polynucleotide nickase comprising an endonuclease which comprises a first subunit and a second subunit and which recognises an asymmetric nucleotide recognition sequence, wherein the first subunit comprises a catalytic domain capable of cleaving one strand of a DNA duplex, and the...

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Bibliographic Details
Main Authors JANULAITIS, ARVYDAS, LUBYS, ARVYDAS, MARKAUSKAS, ALGIMANTAS, STANKEVICIUS, KORNELIJUS
Format Patent
LanguageEnglish
French
German
Published 30.01.2002
Edition7
Subjects
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Summary:A strand-specific polynucleotide nickase comprising an endonuclease which comprises a first subunit and a second subunit and which recognises an asymmetric nucleotide recognition sequence, wherein the first subunit comprises a catalytic domain capable of cleaving one strand of a DNA duplex, and the second subunit is incapable of cleaving the other strand of the DNA duplex.
Bibliography:Application Number: EP20010304891