DIAGNOSTIC METHOD BASED ON THE FUNCTION OF A PROTEIN PRODUCED IN A CELL-FREE ENVIRONEMENT FROM A NUCLEIC ACID SAMPLE

Method for detecting a known function from nucleic acids (NA) in a sample comprises: (1) preparing, from NA, sequences (NA1) comprising the gene(s) (I), encoding proteins (II) corresponding to the function, plus control elements required for their transcription and translation; (2) in vitro transcri...

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Bibliographic Details
Main Authors BLOCH, JEAN-FRANCOIS, DAUTEL, SANDRINE, DUPRET, DANIEL, MASSON, JEAN-MICHEL, LEFEVRE, FABRICE
Format Patent
LanguageEnglish
French
German
Published 04.10.2001
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:Method for detecting a known function from nucleic acids (NA) in a sample comprises: (1) preparing, from NA, sequences (NA1) comprising the gene(s) (I), encoding proteins (II) corresponding to the function, plus control elements required for their transcription and translation; (2) in vitro transcription and translation of NA1; and (3) detection and/or measurement of the function of (II) produced. Independent claims are also included for the following: (1) kits for performing this method; and (2) support, comprising a series of sites for performing the new method, in which each site allows detection and/or measurement of a different function.
Bibliography:Application Number: EP19990957373