METHOD FOR THE EXPONENTIAL AMPLIFICATION OF MOLECULAR MATRICES

• Main Authors: VON KIEDROWSKI, GUENTER, KLUSSMANN, SVEN, FUERSTE, JENS, PETER, KLEIN, THOMAS
• Format: Patent
• Language: English; French; German
• Published: 16.08.2001
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a method for the exponential amplification of molecular matrices, comprising the following steps: (a) binding the molecular matrices to the surface of a solid phase using a reversible linker on the matrix; (b) adding matrix fragments, one of the fragments having an optionally protected linker unit; (c) synthesising copies of the matrix; (d) removing excess matrix fragments and reaction auxiliaries; (e) detaching the copies from the matrices; and (f) populating free binding sites on the solid phase with synthesised matrix copies. The invention also relates to a method for chemical evolution through exponential amplification of molecular matrices, comprising one or more of the following evolution cycles: (1) selection of a subpopulation of molecular matrices from a combinatorial original library; and (2) amplification and mutation of the selective matrices for providing a mutant library, comprising one or more of the amplification cycles (a) to (f) defined above.