Species specific detection of nucleic acids using an analysing element

• Main Authors: KELLER, VOLKER, RAUSCHER, ANDREAS, STEINBISS, JOACHIM, SCHLIPFENBACHER, REINER, KLEPP, JUERGEN
• Format: Patent
• Language: English; French; German
• Published: 16.03.2005
• Edition: 7
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Detecting nucleic acid (I) on an analytical element (AE) that comprises a sample application zone (AZ) and a detection zone (DZ), and allows liquid transport between AZ and DZ, involves applying (I)-containing sample to AZ and detecting (I) in DZ by hybridization with a detectable probe (II). The new feature is that (I) is denatured on AE. Independent claims are also included for the following: (1) AE that is impregnated with a denaturing agent (III) for (I); and (2) a kit for detecting (I) comprising an AE and other reagents for detection.