Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor

• Main Authors: MATSUNAGA, HARUYUKI, OOE, NORIHISA
• Format: Patent
• Language: English; French; German
• Published: 17.12.2003
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The present invention provides an animal cell expressing a gene coding for a ligand-responsive transcription control factor and securely maintaining a DNA comprising in a molecule, the following genes (a) and (b): (a) a reporter gene connected downstream from a transcription control region, in which said transcription control region substantially consists of a recognition sequence of said ligand-responsive transcription control factor and a minimum promoter which can function in said cell; and (b) a selective marker gene which can function in said cell; provided that the following gene (c): (c) a reporter gene connected downstream from a promoter having a transcription activity which is unchanged by having said responsive transcription control factor contacted with a ligand of said ligand-responsive transcription control factor, said reporter gene (c) coding for a protein which can be differentiated from the protein encoded by said gene (a) is not present in said cell and the like. The cell in the present invention is a cryopreservable cell which can be used to measure the action of a chemical substance over the transcription control ability of a ligand-responsive transcription control factor.