CLASS I SEQUENCE BASED TYPING OF HLA-A, -B, AND -C ALLELES BY DIRECT DNA SEQUENCING
There is provided a method for directly typing or sequencing HLA-A, -B, or -C alleles from a tissue sample wherein exons 2 and 3 of the HLA-A, -B, or -C alleles from the sample are amplified together in a locus specific manner and then separated out and individually amplified in a locus specific man...
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Main Authors | , , , |
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Format | Patent |
Language | English French German |
Published |
07.07.2004
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Edition | 7 |
Subjects | |
Online Access | Get full text |
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Summary: | There is provided a method for directly typing or sequencing HLA-A, -B, or -C alleles from a tissue sample wherein exons 2 and 3 of the HLA-A, -B, or -C alleles from the sample are amplified together in a locus specific manner and then separated out and individually amplified in a locus specific manner. After the two amplifications, the amplified exons are directly sequenced, the sequences are recombined, and a comparison is made between the derived HLA allele sequence and an HLA allele database, thereby giving an exact HLA-A, -B, or -C type for the sample being tested. |
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Bibliography: | Application Number: EP19980907404 |