CHRYSOSPORIUM CELLULASE AND METHODS OF USE

• Main Authors: OLSON, PHILIP, T, BEN-BASSAT, ARIE, OKOUNEV, OLEG NICOLAEVICH, EMALFARB, MARK, AARON, BURLINGAME, RICHARD, P, CHERNOGLAZOV, VLADIMIR MIKHAYLOVICH, SINITSYN, ARKADY PANTELEIMONOVICH, SOLOVJEVA, IRINA VLADIMIROVNA
• Format: Patent
• Language: English; French; German
• Published: 23.11.2011
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; APPARATUS THEREFOR; BEER; BIOCHEMISTRY; BLEACHING LEATHER OR FURS; CANDLES; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; DYEING LEATHER, FURS, OR SOLID MACROMOLECULAR SUBSTANCES INANY FORM; DYEING OR PRINTING TEXTILES; ENZYMOLOGY; FATTY ACIDS THEREFROM; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; LAUNDERING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PAPER-MAKING; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; REGENERATION OF PULPING LIQUORS; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; TEXTILES; TREATMENT OF TEXTILES OR THE LIKE; TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking, color brightening, depilling and biobleaching of paper and pulp and treatment of waste streams. The present invention also relates to the isolation and purification of cellulase enzymes, having glucanase and cellobiohydrolase activity, and useful for stonewashing applications.