PURIFICATION OF BIOLOGICALLY ACTIVE PEPTIDES FROM MILK

A method of separating a soluble milk component from milk is disclosed. The method involves the use of tangential flow filtration across a membrane to form a retentate and a permeate, combining the permeate with the original milk sample, and repeating this procedure until the milk has been sufficien...

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Bibliographic Details
Main Authors KUTZKO, JOSEPH, P, SHERMAN, LEE, T, HAYES, MICHAEL, L
Format Patent
LanguageEnglish
French
German
Published 23.06.1999
Edition6
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FOODS OR FOODSTUFFS
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
ORGANIC CHEMISTRY
PEPTIDES
PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
PROTEIN COMPOSITIONS FOR FOODSTUFFS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
TRAPPING
VINEGAR
WINE
WORKING-UP PROTEINS FOR FOODSTUFFS
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Summary:A method of separating a soluble milk component from milk is disclosed. The method involves the use of tangential flow filtration across a membrane to form a retentate and a permeate, combining the permeate with the original milk sample, and repeating this procedure until the milk has been sufficiently purified. Preferably, the milk is combined with a chelating agent, such as EDTA, to improve the purification efficiency. This procedure is advantageously employed with milk from transgenic animals which have been genetically altered to express exogenous proteins, such as therapeutic proteins, in their milk.
Bibliography:Application Number: EP19970923643