Clonong and expression of xylanase genes from fungal origin

Methods and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having xylanase activity and their overexpression in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain stable over a broader pH rang...

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Main Authors VISSER, JACOB, VAN DEN BROECK, HENRIETTE CATHARINA, DE GRAAFF, LEENDERT HENDRIK, HARDER, ABRAHAM, HILLE, JAN DIRK RENE, VAN OOYEN, ALBERT JOHANNES JOSEPH
Format Patent
LanguageEnglish
French
German
Published 20.01.1999
Edition6
Subjects
APPARATUS THEREFOR
BAKERY PRODUCTS
BAKING
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
EDIBLE DOUGHS
ENZYMOLOGY
FODDER
FOODS OR FOODSTUFFS
HUMAN NECESSITIES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PAPER-MAKING
PRESERVATION THEREOF
PROCESSES USING MICROORGANISMS
PRODUCTION OF CELLULOSE
PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REGENERATION OF PULPING LIQUORS
SPIRITS
TEXTILES
THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS
VINEGAR
WINE
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Summary:Methods and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having xylanase activity and their overexpression in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain stable over a broader pH range than do xylanases of bacterial origin. The present invention provides for the high level production of fungal xylanases which are may be used in a variety of industrial applications requiring xylanase activity at a low pH.
Bibliography:Application Number: EP19980114804