METHOD OF CONTROLLED GENETIC TRANSFORMATION OF AN ANIMAL MAMMARY GLAND AND A DEVICE FOR INTRODUCING GENETIC MATERIAL INTO THE MAMMARY DUCT OF AN ANIMAL MAMMARY GLAND

• Main Authors: NIKITIN, VLADIMIR AFANASIEVICH, ROZENKRANTS, ANDREI ALEXANDROVICH, SOBOLEV, ALEXANDR SERGEEVICH
• Format: Patent
• Language: English; French; German
• Published: 07.08.1996
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROMTHE BODY; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS; VINEGAR; WINE

The proposed method of controlled genetic transformation of a mammary gland involves: the introduction of genetic material into the animal's body in a soluble complex with carriers; the controlled delivery of genetic material by the said carriers to the target cell by specific receptor-mediated endocytosis, and whence to the cell nucleus by cytoplasm-nucleus transport. The said soluble complex is introduced into the animal's body via the mammary duct of the mammary gland. The proposed device for introducing genetic material into the mammary duct of an animal's mammary gland comprises a housing (1) with a depression (2) for accommodating the teat of the mammary gland, a central aperture (3) and an annular channel (4) at the base of the depression (2) which can communicate with a vacuum source for widening the teat sphincter. The central aperture (3) is used to introduce the device (6) through which the genetic material is introduced into the mammary duct.