Method for processing and preserving collagen-based tissues for transplantation

• Main Authors: COLEMAN, CHRISTOPHER, NAG, ABHIJIT, NICHOLS, KEN B, GRIFFEY, EDWARD S, LIVESEY, STEPHEN A, DEL CAMPO, ANTHONY A
• Format: Patent
• Language: English; French; German
• Published: 07.12.2005
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; BLASTING; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMBINED HEATING AND REFRIGERATION SYSTEMS; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; DRYING; DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM; ENZYMOLOGY; FISHING; FORESTRY; HEAT PUMP SYSTEMS; HEATING; HUMAN NECESSITIES; HUNTING; HYGIENE; LIGHTING; LIQUEFACTION SOLIDIFICATION OF GASES; MANUFACTURE OR STORAGE OF ICE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MECHANICAL ENGINEERING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PRODUCING, WORKING OR HANDLING ICE; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REFRIGERATION OR COOLING; SPIRITS; TRAPPING; VINEGAR; WEAPONS; WINE

A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.