ANTIFUNGAL POLYPEPTIDE, PROCESS FOR PRODUCING IT

• Main Authors: HILGENFELD, ROLF, WINK, JOACHIM, HAENEL, HEINZ, BRAUN, PETER, LOGEMANN, JUERGEN, ECKES, PETER, ULBRICH, NORBERT, SACHSE, BURKHARD, SCHELL, JOZEF
• Format: Patent
• Language: English; French; German
• Published: 07.04.1993
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Le polypeptide ayant la séquence: Ala-Thr-Tyr-Asn-Gly-Lys-Cys-Tyr-Lys-Lys-Asp-Asn-Ile-Cys-Lys-Tyr-Lys-Ala-Gln-Ser-Gly-Lys-Thr-Ala-Ile-Cys-Lys-Cys-Tyr-Val-Lys-Lys-Cys-Pro-Arg-Asp-Gly-Ala-Lys-Cys-Glu-Phe-Asp-Ser-Tyr-Lys-Gly-Lys-Cys-Tyr-Cys peut être produit par fermentation d'Aspergillus giganteus et est utile comme fongicide. L'expression de ce gène polypeptidique dans des plantes permet d'inhiber fortement la croissance de champignons phytopathogènes sur la plante. The polypeptide with the sequence (SEQ ID NO: 1) - Ala-Thr-Tyr-Asn-Gly-Lys-Cys-Tyr-Lys-Lys-Asp- - Asn-Ile-Cys-Lys-Tyr-Lys-Ala-Gln-Ser-Gly-Lys- - Thr-Ala-Ile-Cys-Lys-Cys-Tyr-Val-Lys-Lys-Cys- - Pro-Arg-Asp-Gly-Ala-Lys-Cys-Glu-Phe-Asp-Ser- - Tyr-Lys-Gly-Lys-Cys-Tyr-Cys - can be produced by the fermentation of Aspergillus giganteus and used as an antifungal agent. Expression of this polypeptide gene in plants strongly inhibits the growth of phytopathogenic fungi on the plant.