A method for enhancing the structural stability of recombinant DNA expression vectors

This invention provides a method of conferring structural stability upon an expression vector which enables the production of a heterologous polypeptide from a host cell. The method comprises lowering the copy number of the expression vector within he host cell to a copy number at which the vectors...

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Bibliographic Details
Main Authors HERSHBERGER, CHARLES LEE, ROSTECK, PAUL ROBERT, JR
Format Patent
LanguageEnglish
French
German
Published 08.07.1992
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:This invention provides a method of conferring structural stability upon an expression vector which enables the production of a heterologous polypeptide from a host cell. The method comprises lowering the copy number of the expression vector within he host cell to a copy number at which the vectors are structurally stable.
Bibliography:Application Number: EP19910311690