NOVEL PEPTIDE SUBSTRATES, METHOD OF PREPARATION AND USE IN C PROTEIN DETERMINATION

• Main Authors: MARTINOLI, JEAN-LUC, QUENTIN, GERARD
• Format: Patent
• Language: English; French; German
• Published: 01.03.1995
• Edition: 5
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

PCT No. PCT/FR90/00973 Sec. 371 Date Oct. 2, 1991 Sec. 102(e) Date Oct. 2, 1991 PCT Filed Dec. 31, 1990 PCT Pub. No. WO91/12268 PCT Pub. Date Aug. 22, 1991.The present invention relates, by way of novel industrial products, to peptide compounds selected from the group consisting of (i) the tripeptides of the formula Y-A1-A2-A3-R(I) in which Y is H or an appropriate group blocking the N-terminal end, A1 is an amino acid residue selected from the group consisting of (2-oxothiazolidin-4-yl)carbonyl [abbreviated to THC], (2-oxotetrahydro-1,3-thiazin-5yl)carbonyl [abbreviated to TZC], thiazolidine-4-carbonyl [abbreviated to ATC] and (tetrahydro-1,3-thiazin-5-yl)carbonyl [abbreviated to AZC] residues, A2 is an amino acid residue selected from the group consisting of Pro, 3Hyp and 4Hyp residues, where the OH side-group of 3Hyp and 4Hyp is capable of being protected by an ether or ester protecting group, A3 is an amino acid residue selected from the group consisting of Arg and Lys residues, and R is a labeling means; and (ii) their addition salts. These novel compounds are useful as substrates in the determination of Protein C.