METHOD AND APPARATUS FOR DETERMINING A PROLIFERATION INDEX OF A CELL SAMPLE

An image processing method and apparatus (10) determines a proliferation index of a cell sample by staining the cells with a chromogen for a proliferation substance and a counterstain for the cell nuclei. The chromogen is activated by an antibody-enzyme conjugate which binds to the proliferation sub...

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Bibliographic Details
Main Author BACUS, JAMES WILLIAM
Format Patent
LanguageEnglish
Published 03.02.1993
Edition5
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:An image processing method and apparatus (10) determines a proliferation index of a cell sample by staining the cells with a chromogen for a proliferation substance and a counterstain for the cell nuclei. The chromogen is activated by an antibody-enzyme conjugate which binds to the proliferation substance to produce a stained cell sample. The stained cell sample is examined with an optical microscope (12), forming a portion of the apparatus, which produces a magnified cell sample image. The apparatus optically filters (18) the cell sample image and produces a pair of optically enhanced proliferation substance and cell nuclei images. The enhanced images are electronically analyzed to determine the amounts of cell nuclei and proliferation substance appearing in the images, respectively. The amounts are then compared to yield a proliferation index for the portion of the cell sample appearing in the cell sample image.
Bibliography:Application Number: EP19900904138