A PROCESS FOR PRODUCING L-PHENYL ACETYL CARBINOL (PAC), AN IMMOBILIZED CELL MASS FOR USE IN THE PROCESS AND A METHOD FOR PREPARING THE CELL MASS

A process for the production of L-phenyl acetyl carbinol (PAC) is carried out with an immobilized cell mass of non-viable cells of a mutant yeast strain that exhibits resistance to aldehyde inhibition during PAC production. Benzaldehyde and a source of pyruvate are reacted in an aqueous medium in th...

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Main Authors YARUS, MICHAEL, J, HEEFNER, DONALD, L, SULLIVAN, SALLY, A, SEELY, ROBERT, J, HAGEMAN, ROBERT, V
Format Patent
LanguageEnglish
Published 21.11.1991
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A process for the production of L-phenyl acetyl carbinol (PAC) is carried out with an immobilized cell mass of non-viable cells of a mutant yeast strain that exhibits resistance to aldehyde inhibition during PAC production. Benzaldehyde and a source of pyruvate are reacted in an aqueous medium in the presence of the immobilized cell mass. Cells in the cell mass contain endogenous pyruvate decarboxylase. Cell walls are chemically crosslinked with polyazetidine and modified to increase permeability to the reactants. A cosolvent for benzaldehyde can be employed to increase PAC production. PAC is useful as an intermediate in the preparation of 1-ephedrine and d-pseudoephedrine, two well-known medicinal chemicals.
Bibliography:Application Number: EP19890912199