PRODUCTION AND PURIFICATION OF RECOMBINANT HUMAN INTERLEUKIN-3 AND MUTEINS THEREOF

Recombinantly produced IL-3 is purified in a series of steps, initially employing hydrophobic interaction, followed by ion-exchange chromatography and gel filtration. Furthermore methods are provided for improved production of recombinant hIL-3 either in glycosylated form from mammalian and yeast ce...

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Bibliographic Details
Main Authors PERSOON, NICOLAAS LUDOVICUS MARIA, VAN LEEN, ROBERT WILLEM
Format Patent
LanguageEnglish
Published 19.06.1991
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:Recombinantly produced IL-3 is purified in a series of steps, initially employing hydrophobic interaction, followed by ion-exchange chromatography and gel filtration. Furthermore methods are provided for improved production of recombinant hIL-3 either in glycosylated form from mammalian and yeast cells or in unglycosylated form from prokaryotes.
Bibliography:Application Number: EP19900200624