ENZYME-LABELED RECEPTOR COMPOSITION, DIAGNOSTIC KIT AND USE IN METHOD TO DETERMINE A LIGAND

An aqueous reagent composition has a pH in the range of from 7 to 9 and comprises: an enzyme-labeled receptor to a ligand of interest, and an organic buffer having a pKa of from 5 to 7 at 25 DEG C which is present in an amount of from 0.1 to 1 molar. This composition can be combined with a compositi...

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Bibliographic Details
Main Authors WARREN, HAROLD CHESTER III, SMITH-LEWIS, MARGARET J, NORKUS, NORBERT SARUNAS
Format Patent
LanguageEnglish
Published 08.04.1992
Edition5
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:An aqueous reagent composition has a pH in the range of from 7 to 9 and comprises: an enzyme-labeled receptor to a ligand of interest, and an organic buffer having a pKa of from 5 to 7 at 25 DEG C which is present in an amount of from 0.1 to 1 molar. This composition can be combined with a composition which provides a dye in the presence of the enzyme and an appropriate substrate in a diagnostic test kit useful for ligand determination. Such determination includes contacting a specimen containing the ligand with the reagent composition to form an immunological complex, separating the complex from other materials, and detecting either the complex or the uncomplexed enzyme-labeled receptor.
Bibliography:Application Number: EP19900300467