DNA CONSTRUCTS CONTAINING A KLUYVEROMYCES ALPHA-FACTOR LEADER SEQUENCE FOR DIRECTING SECRETION OF HETEROLOGOUS DERIVATIVES

DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces alpha -factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis alpha...

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Bibliographic Details
Main Authors BRAKE, ANTHONY J, VAN DEN BERG, JOHANNES A
Format Patent
LanguageEnglish
Published 16.06.1993
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
VINEGAR
WINE
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Summary:DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces alpha -factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis alpha -factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.
Bibliography:Application Number: EP19880201631