PROCESS FOR THE PREPARATION OF DOUBLE STRANDED DNA

It is possible by synthesis of a DNA single strand with a hairpin structure at one or both ends, filling in to give the double strand and opening or elimination of the hairpin structure(s) to obtain even very long double-stranded DNA fragments or genes. It is also possible to attach mutagenic primer...

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Bibliographic Details
Main Authors HEIN, FRIEDRICH, UHLMANN, EUGEN
Format Patent
LanguageEnglish
German
Published 13.09.1989
Edition4
Subjects
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Summary:It is possible by synthesis of a DNA single strand with a hairpin structure at one or both ends, filling in to give the double strand and opening or elimination of the hairpin structure(s) to obtain even very long double-stranded DNA fragments or genes. It is also possible to attach mutagenic primers to the single strand and thus to carry out site-specific mutagenesis. Furthermore, the preparation of highly labelled DNA sequences is possible in the filling-in reaction.
Bibliography:Application Number: EP19880107660