PROCESS FOR THE PREPARATION OF DOUBLE STRANDED DNA

It is possible by synthesis of a DNA single strand with a hairpin structure at one or both ends, filling in to give the double strand and opening or elimination of the hairpin structure(s) to obtain even very long double-stranded DNA fragments or genes. It is also possible to attach mutagenic primer...

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Bibliographic Details
Main Authors HEIN, FRIEDRICH, UHLMANN, EUGEN
Format Patent
LanguageEnglish
German
Published 13.09.1989
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
VINEGAR
WINE
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Summary:It is possible by synthesis of a DNA single strand with a hairpin structure at one or both ends, filling in to give the double strand and opening or elimination of the hairpin structure(s) to obtain even very long double-stranded DNA fragments or genes. It is also possible to attach mutagenic primers to the single strand and thus to carry out site-specific mutagenesis. Furthermore, the preparation of highly labelled DNA sequences is possible in the filling-in reaction.
Bibliography:Application Number: EP19880107660