Production of L-phenyl alanine with the aid of recombinant bacteria

• Main Authors: MARQUARDT, RUDIGER, WOHNER, GERHARD, BRAU, BARBARA, THEN, JOHANN, PRAVE, PAUL, DR
• Format: Patent
• Language: English; French; German
• Published: 09.11.1988
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Fumarsäure, Ammoniumionen und Phenylpyruvat können von genetisch manipulierten Mikroorganismen, die ein Aspartase-Gen sowie ein Aminotransferase-Gen auf einem replizierenden extra-chromosomalen Element oder getrennt auf zwei kompatiblen Plasmiden enthalten, die in dem betreffenden Mikroorganismus exprimiert werden, in hohen Geschwindigkeiten zu L-Phenylalanin transformiert werden. Fumaric acid, ammonium ions and phenylpyruvate can be transformed at high rates into L-phenylalanine by genetically manipulated microorganisms which contain an aspartase gene and an amino- transferase gene on a replicating extrachromosomal element or separately on two compatible plasmids which are expressed in the relevant microorganism.