PYRUVATE OXIDASE HAVING HIGH THERMAL STABILITY, ANALYTICAL METHODS USING IT AND ANALYTICAL REAGENTS CONTAINING IT

• Main Authors: YAMAMOTO, KAZUMI, KIKUCHI, TOSHIRO, EMI, SHIGENORI
• Format: Patent
• Language: English
• Published: 13.10.1993
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A pyruvate oxidase having high thermal stability, being stable at a pH of 7.0 for 10 minutes at a temperature of up to 45 DEG C, can be obtained by culturing a strain of bacteria of the genus Lactobacillus which has pyruvate oxidase activity, such as Lactobacillus sp. TE-6103 (FERM BP-1419). This pyruvate oxidase can be used in analytical methods requiring such an enzyme, for which purpose it can form part of an analytical reagent for use in such a method.