ISOLATION OF UNREDUCED OLIGOSACCHARIDES
A preparative scale method for the isolation of unreduced oligosaccharides of glycoproteins and glycohormones having a N-linked oligosaccharide structure is disclosed. The method employs hydrazinolysis of the glycoprotein or glycohormone under reaction conditions to cause cleavage at the N-linked s...
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Main Authors | , |
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Format | Patent |
Language | English |
Published |
09.10.1991
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Subjects | |
Online Access | Get full text |
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Summary: | A preparative scale method for the isolation of unreduced oligosaccharides of glycoproteins and glycohormones having a N-linked oligosaccharide structure is disclosed. The method employs hydrazinolysis of the glycoprotein or glycohormone under reaction conditions to cause cleavage at the N-linked sites, producing a mixture having as a major component a de-N-acetylated hydrazone derivative of the oligosaccharides, followed by N-acylation of the hydrazone derivative, acid-catalysis of the hydrazone derivative to produce unreduced oligosaccharides, and subjecting the resulting unreduced oligosaccharides to cellulose column chromatography to remove contaminants and to recover the unreduced oligosaccharides. |
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Bibliography: | Application Number: EP19860870123 |