INTACT GENE AND METHOD OF EXCISING AND CLONING SAME

The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with a nuclease and a denaturing agent under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of suc...

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Bibliographic Details
Main Authors MCCUTCHAN, THOMAS F, DAME, JOHN BARTON
Format Patent
LanguageEnglish
Published 14.08.1991
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with a nuclease and a denaturing agent under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products such as m-RNA, S-RNA, t-RNA, polypeptides and the like.
Bibliography:Application Number: EP19850108829