Monoclonal antibodies, processes for their preparation and methods for their use

• Main Authors: YOSHIDA, HAJIME, FUJIYOSHI, NOBUO
• Format: Patent
• Language: English; French; German
• Published: 26.09.1984
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Monoclonal antibodies specific for unglycosylated human interferon- beta are prepared by immunizing a mammal with unglycosylated human interferon- beta to produce cells capable of producing antibodies in the body of said mammal, fusing said cell with lined myeloma cells originating from a mammal other than the immunized mammal, selecting the resultant fused cells for the production of antibodies specific for unglycosylated human interferon- beta , subjecting the selected fused cells to cloning to produce monoclones, selecting the resultant monoclones for reactivity with at least one member selected from unglycosylated human interferon- beta and other human interferons, culturing the selected monoclones, and recovering the desired monoclonal antibody from the culture thereby obtained. Such monoclonal antibodies specific for unglycosylated human interferon- beta are used in processes for the purification and quantitative determination of unglycosylated human interferon- beta .