Process for isolating tetracyclines

The subject of this invention is a method for isolating tetracyclines from the broth after the biosynthesis or from the mother liquor after isolating crude bases by means of ion-exchange and sorption resins. The broth or the acid mother liquor are passed through a bed of slightly alkaline anion-exch...

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Main Authors MICHALSKA, LUCYNA, M.SC, CZARNY, KAZIMIERZ, M.SC, STASIAK, MARIA, M.SC, MUCHA, BARBARA, M.SC, HEROPOLITANSKI, RYSZARD, SOCHACKA, JOLANTA, M.SC
Format Patent
LanguageEnglish
French
German
Published 26.10.1983
Subjects
ACYCLIC OR CARBOCYCLIC COMPOUNDS
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
SPIRITS
VINEGAR
WINE
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Summary:The subject of this invention is a method for isolating tetracyclines from the broth after the biosynthesis or from the mother liquor after isolating crude bases by means of ion-exchange and sorption resins. The broth or the acid mother liquor are passed through a bed of slightly alkaline anion-exchange resin with the functional groups blocked by a mineral acid anion. The antibiotic containing eluate is adsorbed on the bed of macroporous resin and then desorbed by washing with a 15-60% aqueous acidified solution af acetone or of methanol. The pure compound is isolated from the eluate by a well-known method. The beds of anion-exchange and sorption resins are regenerated with an aqueous solution of acetone or of methanol containing to 80% by volume of the organic solvent with addition of 0,4-6% by weight of sodium or potassium hydroxide or of ammonia.
Bibliography:Application Number: EP19830103663