HIGH AFFINITY AND AGGREGATIVELY STABLE ANTIBODIES ON THE BASIS OF VARIABLE DOMAINS VL AND A DERIVATIVE VHH

• Main Authors: SOLOVIEV, Valeriy Vladimirovich, NEMANKIN, Timofey Aleksandrovich, MOROZOV, Dmitriy Valentinovich, CHERNYKH, Yulia Sergeevna, GONCHAROVA, Olga Vladimirovna, CHERNOVSKAYA, Tatyana Veniaminovna, SOFRONOVA, Ekaterina Vladimirovna, ULITIN, Andrey Borisovich, IVANOV, Roman Alexeevich, EKIMOVA, Victoria Mikhailovna, EVDOKIMOV, Stanislav Rudolfovich, USTYUGOV, Yakov Yurevich, KORZHAVIN, Dmitriy Valerievich
• Format: Patent
• Language: English; Russian
• Published: 04.02.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Monoclonal IgG-type antibodies were suggested comprising variable domains represented by a combination of VHH-derivative with a variable domain of the light chain VL. Said antibodies can comprise amino acid substitutions at positions 44 and/or 45 (Kabat numbering) or combinations thereof. The antibodies of the invention possess increased affinity and improved aggregation stability. Предложены моноклональные антитела типа IgG, содержащие вариабельные домены в виде комбинации производного VHH с вариабельным доменом легкой цепи VL. Указанные антитела могут содержать замены аминокислот в позициях 44 и/или 45 (по нумерации по Kabat) или их комбинации. Описанные антитела обладают повышенной аффинностью и улучшенной агрегационной стабильностью.